When choosing a valve, always pay close attention to its advertised leakage rate. Confirm this with your valve manufacturer as not all publish the leak specs for all components. Many valves have leak rates of 15-100 sccm, which can be fine for many applications. Life Science applications often utilize on board pressure or vacuum pumps and storage accumulators. A good design practice is to reduce the on/off cycles of the pumps to reduce noise, vibration etc. These applications call for low leak valves in the 0.1-1.0 sccm range when possible.
It is widely accepted that "bubble-tight" refers to a valve that, when fully closed, does not pass any bubbles when one side of the valve seat is pressurized with air/nitrogen, and the other side of the seat is submerged in water. Actual test procedures can vary from manufacturer to manufacturer. There is no formal standard to define bubble-tight.
For example, most tires are tested to 1x10-5atm-cc/s, units commonly seen from a high vacuum helium leak detector. On an air pressure decay style leak detector, the equivalent leak rate would typically be stated as 0.0002 sccm. And a bubble leak test would take 10 minutes before the first bubble would escape to the atmosphere.
Low leak valves feature large cross sectional O-rings, minimal leak points, and proven poppet designs. All mounting hardware is outside of the flow path and no internal parts are threaded during assembly to reduce the possibility of contamination.