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Life Science Compatibility Chart
Listing several different types of chemicals typically used within the Life Science community and their interactions with the most commonly used types of plastics.
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| Life Science Common Reagents & Chemicals | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| What's in it: | Common Applications: | ||||||||||||||||||||||||
| Buffers | |||||||||||||||||||||||||
| Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes. Buffers are an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa. Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications. | ABS | Acrylic | COP | CPVC | Delrin/Acetal | HDPE | Noryl | Nylon 66 | PBT | PC | PEEK | PES | PET | Polysulfone | PP | PPS | PTFE | PVC | PVDF | Torlon | UHMW | Ultem | |||
| Tris-Buffered Saline (TBS) | Tris(hydroxymethyl)aminomethane (tris)-buffered saline | Used to dilute substances used in laboratory experiments. Often used in immuno-blotting for both membrane washing and antibody dilution | |||||||||||||||||||||||
| Tris-EDTA (TE) | 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid | Tris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer preparation. | |||||||||||||||||||||||
| Tris-Acetate-EDTA Buffer | |||||||||||||||||||||||||
| HEPES Buffered Saline (HBS) | (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) | Widely used in cell culture, largely because it is better at maintaining physiological pH. | |||||||||||||||||||||||
| Trizma (Tris base) | Tris(hydroxymethyl)aminomethane | Frequently used buffer in cell and molecular biology experiments. Used to increase permeability of cell membranes. It is a component of the Moderna COVID-19 vaccine. | |||||||||||||||||||||||
| Denhardt’s Solution | 0 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP). | block non-specific binding sites in western, northern and Southern blot membranes | |||||||||||||||||||||||
| Bicine buffer | |||||||||||||||||||||||||
| Phosphate Buffered Saline, PBS | PBS contains 3.2 mM Na2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 137 mM NaCl, pH 7.4. | suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions. | |||||||||||||||||||||||
| Dulbecco’s Phosphate Buffered Saline (DPBS) | |||||||||||||||||||||||||
| Annexin V Binding Buffer | |||||||||||||||||||||||||
| Sodium Citrate Solution | 1M sodium citrate in H2O | A to 100% to 190F | A to 70F | A to 70F | A to 70F | A to 70F | A to 70F | A to 70F | A to 70F | AB to 140F | A to 140F | A to 70F | |||||||||||||
| Sodium Acetate Solution | *Also see Sodium Acetate below | B at 70F | A to 140F | A to 225F no stress | A to 100% to 180F | A to 100% to 160F, no stress | A to 130F | AB to 100% to 80F | A to 130F | A to 70F | A to 100% to 70F | A to 70F | A to 100% to 80F | A to 100% to 200F | A to 100% to 225F no stress | A to 100% to 200F | A to 100% to 500F | A to 100% to 160F no stress | A to 100% to 275F | A to 60% to 70F | A to 100% to 140F | A solution to 70F | |||
| Detergents | |||||||||||||||||||||||||
| There are many types of detergents used in biological applictions and research. Applications range from cell lysis, to protein denaturation to cleaning steps in equipment. | |||||||||||||||||||||||||
| SDS | Sodium Dodecyl Sulfate | Extraction of DNA and RNA, Solubilization of specimens for diagnostic applications, Cell lysis | |||||||||||||||||||||||
| Liposome preparation | Prevention of reagent and analyte precipitation from solution | Prevention of non-specific binding in immunoassays" | |||||||||||||||||||||||
| Triton™ X-114 | Polyethylene glycol tert-octylphenyl ether | Used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C). | |||||||||||||||||||||||
| Triton™ X-100 | Polyethylene glycol tert-octylphenyl ether | In the permeabilization of cells for immunofluorescence staining | |||||||||||||||||||||||
| CTAB | Cetyltrimethylammonium bromide | One of the main components of some buffers for the extraction of DNA | |||||||||||||||||||||||
| CHAPS | 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate | Useful for membrane protein solubilization when it is important to maintain protein activity | |||||||||||||||||||||||
| General Bioreagents (Acid/Base) | |||||||||||||||||||||||||
| Many of these may be used in buffer solutions. | |||||||||||||||||||||||||
| DMSO | Dimethyl Sulfoxide | Used in polymerase chain reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, transfection protocols | NR at 70F | A | NR at 70F | NR at 70F | A to 122F | A to 200F | A to 70F | C at 70F | NR at 70F | B to 212F | NR at 70F | NR at 70F | A to 125 F | A to 200F | A to 500 F | NR at 70F | NR 100% at 70F | SWELLS | |||||
| Ethanol Solution | Used in the purification and precipitation of biomolecules, in staining and restaining specimens in histology, in dehydrating tissues before embedding, and in disinfection. | AB to 70F no stress | NR to 100% to 70F C to 70% to 70F A to 50% to 70F | A | A to 225F no stress | A to 100% to 140F | A to 100% to 160F no stress | A to 100% to 80F | A to 100% to 70F | A to 140F | A to 90% to 90F AB to 100% to 70F B to 100% to 120F | A to 100%to 212F | AB to 70F | A to 100% to 70F | B to 100% to 120F | A to 100% to 225F | A to 100% to 300F | A to 100% at 500F | A to 100% 270F | A to 100% to 176F | A to 70F | A to 100% to 170F | A to 70F | ||
| Sodium hypochlorite | "liquid bleach" | Inhibits microbial growth | NR to 70F | A to 100% to 140F | A to 15% to 140F | NR to 5% to 70F | A to 100% to 160F | AB to 100% to 200F | NR to 5% to 70F | A to 10% to 70F | AB to 100% to 70F | AB to 100% to 212F | B to 100% to 70F | A to 5% to 70F NR to 100% to 70F | A to 100% to 200F | C to 14% to 70F | NR to 100% to 70F | A to 100% to 500F | A to 12.5% to 140F | A to 17% to 280F | A to 15% to 70F | A to 100% to 170F | NR to 5% to 70F | ||
| Hydrogen peroxide | Inhibits microbial growth | NR at 100% at 70F A to 10% to 70F | A to 63% to 100F | A | NR to 100% at 70F | NR at 70F | AB to 100% to 70F | NR at 100% to 70F | NR at 70F | A to 90%to 130F B at 100% at 70F | A to 100% to 125F | A to 100% to 212F | A to 100% to 70F | A to 90% to 80F | A to 100% 10 70F | NR at 100% at 70F | NR to 50-100% at 70F | A to 100% to 500F | AB to 100% to 100F | A to 100% to 200F | A to 100% to 70F | A to 90% to 70F | |||
| Sodium Acetate | sodium salt of acetic acid | Sodium acetate is used as the carbon source for culturing bacteria. Sodium acetate is also useful for increasing yields of DNA isolation by ethanol precipitation. | B at 70F | A to 140F | A to 225F no stress | A to 100% to 180F | A to 100% to 160F, no stress | A to 130F | AB to 100% to 80F | A to 130F | A to 70F | A to 100% to 70F | A to 70F | A to 100% to 80F | A to 100% to 200F | A to 100% to 225F no stress | A to 100% to 200F | A to 100% to 500F | A to 100% to 160F no stress | A to 100% to 275F | A to 60% to 70F | A to 100% to 140F | A solution to 70F | ||
| Imidazole | Imidazole can be used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column. Used in buffer solutions. | ||||||||||||||||||||||||
| Urea Solution | Carbamide | commonly used for sample preparation prior to | |||||||||||||||||||||||
| electrophoretic methods. Urea is also used to facilitate enzymatic digestion of proteins for analysis by mass spectrometry." | B at 70F | A | A to 100% to 225F no stress | A to 100% to 70F | A to 100% to 160F | A to 150F | A to 100% to 70F | NR at 70F | NR at 70F | A to 212F | AC at 70F | C at 70F | A to 100% to 225F no stress | A to 100% to 200F | A to 100% to 500F | A to 100% to 160F no stress | A to 100% to 250F | A to 70F | |||||||
| 10M Sodium Hydroxide (NaOH) | Used for many applications including adjusting the pH of various solutions. | AB to 100% to 70F | NR at 70F | A to 100% to 150F | NR at 70F | A to 100% to 140F | A to 100% to 185F | A to 100% to 70F | NR at 70F | NR at 70F | A to 100% to 70F | A to 54% to 70F | NR at 70F | A to 50F to 125F | A to 100% to 125F | A to 100% to 70F | A to 100% to 480F | A to 100% to 140F | NR at 70F | NR at 70F | A to 100% to 170F | NR at 70F | |||
| Potassium Salt | A to 140F | A to 225F no stress | A to 70F | A to 140F | A to 150F | A to 70F | A to 70F | A to 70F | A to 212F | A to 70F | A to 70F | A to 200F | A to 100% to 150F | A to 200F | A to 500F | A to 100% to 160F | A to 100% to 275F | A to 70F | A to 70F | ||||||
| Potassium Chloride | A to 100% to 70F | A to 100F to 140F | A to 100% to 225F | A to 100% to 180F | A to 100% to 160F no stress | A to 100% to 150F | A to 100% to 70F | A to 100% to 80F | A to 100% to 70F | A to 100% to 212F | A to 80F | A to 200F | A to 100% to 225F | A to 100% to 200F | A to 100% to 500F | A to 100% to 160F | A to 100% to 275F | A to 90% to 70F | A to 70F | ||||||
| Potassium Phosphate | |||||||||||||||||||||||||
| Sodium Chloride | A to 100% to 70F | A to 100% 140F | A | A to 100% to 225F no stress | A to 100% to 70F | A 100% to 160F | A to 100% to 200F | A to 100% to 70F | A to 100% to 80F | A to 100% to 120F | A to 100% to 212F | A to 70F | A to 100% to 70F | A to 100% to 200F | A to 100% to 225F | A to 100% to 200F | A to 100% to 500F | A to 100% to 160F | A to 100% to 275F | A to 100% to 70F | A to 170F | A to 70F | |||
| Zinc Chloride | Used in water treatment, dry cell batteries, | A to 100% to 70F | A to 100% to 70F | A to 225F no stress | NR to 100% to 70F | A to 100% to 180F | A to 70F | NR to 100% to 70F | A to 100% to 80F | A to 140F | A to 100% to 212F | A to 80F | A to 300F | A to 100% to 225F no stress | A to 100% to 200F | A to 100% to 500F | A to 100% to 160F no stress | A to 100% to 275F | A to 170F | A to 10% to 70F | |||||
| HCL | Used as a standard and as a strong acid for pH maintenance | BC to 100% to 70F | NR at 70F | A | NR to 75-100% 176F | NR to 100% at 70F | A to 100% to 140F | aA to 100% to 180F | NR to 100% to 70F | NR to 100% to 70F | NR at 70F | A to 100% to 212F | A to 60% to 140F | NR to 100% at 70F | A to 100% to 70F | A to 100% to 70F | NR at 70F | A to 100% to 500F | NR at 100% to 70F | A to 50% to 175F | AB to 37% to 200F | A to 100% to 140F | A to 100% to 70F | ||
| Disodium Phosphate Solution | Buffer component | A to 235F no stress | A to 140F | A to 160F | A to 225F no stress | A to 500F | A to 140F | AB to 100% to 280F | |||||||||||||||||
| Methanol | A | ||||||||||||||||||||||||
| Octanol | |||||||||||||||||||||||||
| Propanol | A | ||||||||||||||||||||||||
| Monosodium Phosphate Solution (1M) | Buffer component | ||||||||||||||||||||||||
| MISC | |||||||||||||||||||||||||
| Gold nanoparticles | Unique optoelectronic properties have been researched and utilized in high technology applications such as organic photovoltaics, sensory probes, therapeutic agents, drug delivery in biological and medical applications, electronic conductors and catalysis. | ||||||||||||||||||||||||
| Proclin 300 | 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) and | ||||||||||||||||||||||||
| 2-Methyl-4-isothiazolin-3-one (MIT)" | Biocide to prevent microbial growth | ||||||||||||||||||||||||
| Tween 20 | Polysorbate-type nonionic surfactant | Many biological processes, surfactant | A | ||||||||||||||||||||||
| EDTA Solution (0.5M)" | 0.5M EDTA, pH 8.0 is an aqueous solution prepared by dissolving Na2EDTA•2H| Suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions. | | |||||||||||||||||||||||
| DTT Solution (1M) | Dithiothreitol | Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. By reducing the disulfide bonds in a protein sample, the protein can be more effectively fragmented and analyzed. | |||||||||||||||||||||||
| Useful : https://www.coleparmer.com/Chemical-Resistance |
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