Life Science Focused Chemical Compatibility

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Life Science Common Reagents & Chemicals
What's in it:Common Applications:
Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes. Buffers are an aqueous solution consisting of a mixture of a weak acid and its conjugate base, or vice versa. Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical applications.ABSAcrylicCOPCPVCDelrin/AcetalHDPENorylNylon 66PBTPCPEEKPESPETPolysulfonePPPPSPTFEPVCPVDFTorlonUHMWUltem
Tris-Buffered Saline (TBS)Tris(hydroxymethyl)aminomethane (tris)-buffered salineUsed to dilute substances used in laboratory experiments. Often used in immuno-blotting for both membrane washing and antibody dilution
Tris-EDTA (TE)2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acidTris and EDTA are two major components which are used throughout the DNA extraction protocol. Tris and EDTA are applicable in lysis buffer preparation, elution buffer preparation and washing buffer preparation.
Tris-Acetate-EDTA Buffer
HEPES Buffered Saline (HBS)(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)Widely used in cell culture, largely because it is better at maintaining physiological pH.
Trizma (Tris base)Tris(hydroxymethyl)aminomethaneFrequently used buffer in cell and molecular biology experiments. Used to increase permeability of cell membranes. It is a component of the Moderna COVID-19 vaccine.
Denhardt’s Solution0 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).block non-specific binding sites in western, northern and Southern blot membranes
Bicine buffer
Phosphate Buffered Saline, PBSPBS contains 3.2 mM Na2HPO4, 0.5 mM KH2PO4, 1.3 mM KCl, 137 mM NaCl, pH 7.4.suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions.
Dulbecco’s Phosphate Buffered Saline (DPBS)
Annexin V Binding Buffer
Sodium Citrate Solution1M sodium citrate in H2OA to 100% to 190FA to 70FA to 70FA to 70FA to 70FA to 70FA to 70FA to 70FAB to 140FA to 140FA to 70F
Sodium Acetate Solution*Also see Sodium Acetate belowB at 70FA to 140FA to 225F no stressA to 100% to 180FA to 100% to 160F, no stressA to 130FAB to 100% to 80FA to 130FA to 70FA to 100% to 70FA to 70FA to 100% to 80FA to 100% to 200FA to 100% to 225F no stressA to 100% to 200FA to 100% to 500FA to 100% to 160F no stressA to 100% to 275FA to 60% to 70FA to 100% to 140FA solution to 70F
Detergents
There are many types of detergents used in biological applictions and research. Applications range from cell lysis, to protein denaturation to cleaning steps in equipment.
SDSSodium Dodecyl SulfateExtraction of DNA and RNA, Solubilization of specimens for diagnostic applications, Cell lysis
Liposome preparationPrevention of reagent and analyte precipitation from solutionPrevention of non-specific binding in immunoassays"
Triton™ X-114Polyethylene glycol tert-octylphenyl etherUsed in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
Triton™ X-100Polyethylene glycol tert-octylphenyl etherIn the permeabilization of cells for immunofluorescence staining
CTABCetyltrimethylammonium bromideOne of the main components of some buffers for the extraction of DNA
CHAPS3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonateUseful for membrane protein solubilization when it is important to maintain protein activity
General Bioreagents (Acid/Base)
Many of these may be used in buffer solutions.
DMSODimethyl SulfoxideUsed in polymerase chain reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, transfection protocolsNR at 70FANR at 70FNR at 70FA to 122FA to 200FA to 70FC at 70FNR at 70FB to 212FNR at 70FNR at 70FA to 125 FA to 200FA to 500 FNR at 70FNR 100% at 70FSWELLS
Ethanol SolutionUsed in the purification and precipitation of biomolecules, in staining and restaining specimens in histology, in dehydrating tissues before embedding, and in disinfection.AB to 70F no stressNR to 100% to 70F C to 70% to 70F A to 50% to 70FAA to 225F no stressA to 100% to 140FA to 100% to 160F no stressA to 100% to 80FA to 100% to 70FA to 140FA to 90% to 90F AB to 100% to 70F B to 100% to 120FA to 100%to 212FAB to 70FA to 100% to 70FB to 100% to 120FA to 100% to 225FA to 100% to 300FA to 100% at 500FA to 100% 270FA to 100% to 176FA to 70FA to 100% to 170FA to 70F
Sodium hypochlorite"liquid bleach"Inhibits microbial growthNR to 70FA to 100% to 140FA to 15% to 140FNR to 5% to 70FA to 100% to 160FAB to 100% to 200FNR to 5% to 70FA to 10% to 70FAB to 100% to 70FAB to 100% to 212FB to 100% to 70FA to 5% to 70F NR to 100% to 70FA to 100% to 200FC to 14% to 70FNR to 100% to 70FA to 100% to 500FA to 12.5% to 140FA to 17% to 280FA to 15% to 70FA to 100% to 170FNR to 5% to 70F
Hydrogen peroxideInhibits microbial growthNR at 100% at 70F A to 10% to 70FA to 63% to 100FANR to 100% at 70FNR at 70FAB to 100% to 70FNR at 100% to 70FNR at 70FA to 90%to 130F B at 100% at 70FA to 100% to 125FA to 100% to 212FA to 100% to 70FA to 90% to 80FA to 100% 10 70FNR at 100% at 70FNR to 50-100% at 70FA to 100% to 500FAB to 100% to 100FA to 100% to 200FA to 100% to 70FA to 90% to 70F
Sodium Acetatesodium salt of acetic acidSodium acetate is used as the carbon source for culturing bacteria. Sodium acetate is also useful for increasing yields of DNA isolation by ethanol precipitation.B at 70FA to 140FA to 225F no stressA to 100% to 180FA to 100% to 160F, no stressA to 130FAB to 100% to 80FA to 130FA to 70FA to 100% to 70FA to 70FA to 100% to 80FA to 100% to 200FA to 100% to 225F no stressA to 100% to 200FA to 100% to 500FA to 100% to 160F no stressA to 100% to 275FA to 60% to 70FA to 100% to 140FA solution to 70F
ImidazoleImidazole can be used to elute tagged proteins bound to nickel ions attached to the surface of beads in the chromatography column. Used in buffer solutions.
Urea SolutionCarbamidecommonly used for sample preparation prior to
electrophoretic methods. Urea is also used to facilitate enzymatic digestion of proteins for analysis by mass spectrometry."B at 70FAA to 100% to 225F no stressA to 100% to 70FA to 100% to 160FA to 150FA to 100% to 70FNR at 70FNR at 70FA to 212FAC at 70FC at 70FA to 100% to 225F no stressA to 100% to 200FA to 100% to 500FA to 100% to 160F no stressA to 100% to 250FA to 70F
10M Sodium Hydroxide (NaOH)Used for many applications including adjusting the pH of various solutions.AB to 100% to 70FNR at 70FA to 100% to 150FNR at 70FA to 100% to 140FA to 100% to 185FA to 100% to 70FNR at 70FNR at 70FA to 100% to 70FA to 54% to 70FNR at 70FA to 50F to 125FA to 100% to 125FA to 100% to 70FA to 100% to 480FA to 100% to 140FNR at 70FNR at 70FA to 100% to 170FNR at 70F
Potassium SaltA to 140FA to 225F no stressA to 70FA to 140FA to 150FA to 70FA to 70FA to 70FA to 212FA to 70FA to 70FA to 200FA to 100% to 150FA to 200FA to 500FA to 100% to 160FA to 100% to 275FA to 70FA to 70F
Potassium ChlorideA to 100% to 70FA to 100F to 140FA to 100% to 225FA to 100% to 180FA to 100% to 160F no stressA to 100% to 150FA to 100% to 70FA to 100% to 80FA to 100% to 70FA to 100% to 212FA to 80FA to 200FA to 100% to 225FA to 100% to 200FA to 100% to 500FA to 100% to 160FA to 100% to 275FA to 90% to 70FA to 70F
Potassium Phosphate
Sodium ChlorideA to 100% to 70FA to 100% 140FAA to 100% to 225F no stressA to 100% to 70FA 100% to 160FA to 100% to 200FA to 100% to 70FA to 100% to 80FA to 100% to 120FA to 100% to 212FA to 70FA to 100% to 70FA to 100% to 200FA to 100% to 225FA to 100% to 200FA to 100% to 500FA to 100% to 160FA to 100% to 275FA to 100% to 70FA to 170FA to 70F
Zinc ChlorideUsed in water treatment, dry cell batteries,A to 100% to 70FA to 100% to 70FA to 225F no stressNR to 100% to 70FA to 100% to 180FA to 70FNR to 100% to 70FA to 100% to 80FA to 140FA to 100% to 212FA to 80FA to 300FA to 100% to 225F no stressA to 100% to 200FA to 100% to 500FA to 100% to 160F no stressA to 100% to 275FA to 170FA to 10% to 70F
HCLUsed as a standard and as a strong acid for pH maintenanceBC to 100% to 70FNR at 70FANR to 75-100% 176FNR to 100% at 70FA to 100% to 140FaA to 100% to 180FNR to 100% to 70FNR to 100% to 70FNR at 70FA to 100% to 212FA to 60% to 140FNR to 100% at 70FA to 100% to 70FA to 100% to 70FNR at 70FA to 100% to 500FNR at 100% to 70FA to 50% to 175FAB to 37% to 200FA to 100% to 140FA to 100% to 70F
Disodium Phosphate SolutionBuffer componentA to 235F no stressA to 140FA to 160FA to 225F no stressA to 500FA to 140FAB to 100% to 280F
MethanolA
Octanol
PropanolA
Monosodium Phosphate Solution (1M)Buffer component
MISC
Gold nanoparticlesUnique optoelectronic properties have been researched and utilized in high technology applications such as organic photovoltaics, sensory probes, therapeutic agents, drug delivery in biological and medical applications, electronic conductors and catalysis.
Proclin 3005-Chloro-2-methyl-4-isothiazolin-3-one (CMIT) and
2-Methyl-4-isothiazolin-3-one (MIT)"Biocide to prevent microbial growth
Tween 20Polysorbate-type nonionic surfactantMany biological processes, surfactantA
EDTA Solution (0.5M)"0.5M EDTA, pH 8.0 is an aqueous solution prepared by dissolving Na2EDTA•2HSuitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions.
DTT Solution (1M)DithiothreitolDithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. By reducing the disulfide bonds in a protein sample, the protein can be more effectively fragmented and analyzed.
Useful : https://www.coleparmer.com/Chemical-Resistance

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